Methanol is a polar-protic solvent, whereas acetonitrile is a polar-aprotic solvent and possesses a stronger dipole moment. This means that the organic modifier used in the mobile phase can have a powerful effect on chromatographic selectivity
Methanol can be used as a mobile phase in reversed-phase liquid chromatography. Methanol (MeOH) has been used in the wholemount immunofluorescence studies of embryonic tissues. As solvent for the preparation of extracts of hyphae of Aspergillus for the estimation of gliotoxin by reversed phase-HPLC.
Methanol can be used as a mobile phase in reversed-phase liquid chromatography. Methanol (MeOH) has been used in the wholemount immunofluorescence studies of embryonic tissues.
| Physical state | Liquid. [Colorless.] |
| Flash point | Open cup: 15.85°C (60.5°F) |
| Auto-ignition temperature | 464°C (867.2°F) |
| Flammable limits |
Lower: 6% Upper: 36.5% |
| Odor | Characteristic. Alcohol-like |
| Molecular weight | 32.05 g/mole |
| Molecular formula | C-H4-O |
| pH | Not available |
| Boiling/condensation point | 64.5°C (148.1°F) |
| Melting/freezing point | -97.8°C (-144°F) |
| Relative density | 0.792 |
| Vapor pressure | 12.9 kPa (97 mm Hg) |
| Vapor density | 1.11 [Air = 1] |
| Volatility | 99.9% (v/v) |
| Odor threshold | 100 ppm |
| Solubility | Soluble in the following materials: water |
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